Extended Data Fig. 1: Characterization of weight loss induced by a cysteine-deficient diet and comparative analysis with other EAA deficiencies.
From: Unravelling cysteine-deficiency-associated rapid weight loss
a, Het and b, KO weight curves with various essential amino acid deficiencies (n ≥ 4 per group). c-e, KO on various essential amino acid deficiencies by class: c, Branched chain amino acids; d, Aromatic amino acids; e, Other EAAs. f, Weight curves of WT compared to Cse Het and KO mice on No Cys diet. Weight loss curves of Cse KO on No Cys plotted individually by g, percentage h, mass. i, Weight loss curves by percentage plotted according to different starting weights (n ≥ 5 in all groups). j, Percentage weight loss at Day 7 of Cse KO mice on a No Cys diet, plotted against starting mass. k, Weight loss curves of male vs female Cse KO mice on a No Cys diet (n = 9 per group). l, Weight curves of female Het and KO mice on a No Cys diet with either 0.4% NAC or 1% GSH in drinking water (n ≥ 4 per group). m-o, Levels of various nutrients in the administered diet (yellow) and in Het (blue) or KO (red) stool from microbiota-depleted mice fed on a No Cys diet for 3 days. m, Amino acid levels. n, Vitamins and palmitic acid levels. o, Glucose levels. p, Weight curves of female mice on a No Cys diet with or without daily injections of 40 mg/kg of GYY4137 (n ≥ 4 per group). q, Weight curves of female mice on a No Cys diet with and without antibiotic treatment (1 g/L Ampicillin) (n ≥ 4 per group). r, Weight curves of Cse KO mice on a No Met No Cys compared to No Cys, No Met, or control diets (n = 4 per group). Hets and KOs mice were co-housed for each experimental condition. Data are mean ± s.d.
