Extended Data Fig. 5: Freezing behavior is encoded in dmPFC neural activity.
From: Prefrontal encoding of an internal model for emotional inference
(a) Decoding accuracy for distinguishing freezing from non-freezing behavior averaged from each rat in the paired group during auditory (red), visual (blue) CSs and shuffled dataset (grey) in recall. (b) Median decoding accuracy of freezing from dmPFC population activity for experimental (colored bars) and shuffled data (grey bars) during auditory (left) and visual (right) CSs in Recall. Dots represent individual animal values (filled = male, open = female). Mann-Whitney U test (two-sided); ** (left): r = 57, p = 0.0022; ** (right): r = 57, p = 0.0022. (c, d) Left: Peri-event time histograms showing normalized calcium activity around freezing periods during auditory (c) and visual (d) CSs for all activated neurons during recall. SEM=shaded. Right: Box plots showing median (center), 25%/75% interquartile range (bounds of box) and extreme data points (whiskers) for calcium responses before and after (green shading in c, d) freezing onset. Mann-Whitney U test (two-sided), c, ***: r = 103447.5, z = 4.42, p = 1.0 × 10−5; d, **: r = 98567, z = 3.07, p = 0.0022; (e) Calcium response (normalized by subtraction of means) to auditory stimulus and SEM (shaded) around the onset of freezing behaviours for co-responsive neurons (defined by activity during recall). (f) Similar to e, but for auditory-only neurons. (g) Similar to e, but for visual-only neurons.
