Extended Data Fig. 14: Freezing related neural activity of amygdala projecting neurons and lack of auditory and visual CS representational merging after conditioning.
From: Prefrontal encoding of an internal model for emotional inference
(a, b) Box plots showing median (center), 25%/75% interquartile range (bounds of box) and extreme data points (whiskers) for auditory CS-evoked calcium responses in paired and unpaired groups taken from time periods during auditory CSs when rats were not freezing. Mann-Whitney U test (two-sided); ***: r = 35608, z = 4.73, p = 2.2 × 10−6; n.s.: r = 27464, z = 0.95, p = 0.34; (c) Decoding accuracy of freezing from non-freezing behavior averaged from each rat in the paired group during auditory (red), visual (blue) CSs and shuffled dataset (grey) in recall. (d) Median decoding accuracy (calculated for each rat) of freezing from the activity of amygdala projecting neurons for experimental and shuffled data during auditory (left) and visual (right) CSs in recall. Dots represent individual animal values (filled = male, open = female). Mann-Whitney U test (two-sided); *** (left): r = 77, p = 5.8 × 10−4; *** (right): r = 77, p = 5.8 × 10−4. (e, f) Left: Peri-event time histogram showing normalized calcium activity around freezing periods occurring during auditory (e) and visual (f) CSs for all activated neurons during recall. SEM = shaded. Right: Box plots showing median (center), 25%/75% interquartile range (bounds of box) and extreme data points (whiskers) for calcium responses before and after (green shading in e, f) freezing onset. Mann-Whitney U test (two-sided), e, **: r = 34850.5, z = 3.17, p = 0.0015; f, n.s.: r = 25543, z = 1.01, p = 0.31. (g, h) Similar to e, f, but for calcium responses around the offset of freezing. g: Mann-Whitney U test (two-sided); n.s.: r = 32518, z = 0.77, p = 0.44; h: Mann-Whitney U test (two-sided); n.s.: r = 25516, z = 0.98, p = 0.33. (i) The Euclidean angular distance (θ) of auditory and visual stimulus evoked neural population vectors for the paired (left) and unpaired (right) groups during preconditioning (PC, top) and recall (bottom) in amygdala projecting dmPFC neurons. (j) The distribution of angular distance between auditory (A) and visual (V) stimulus evoked population vectors in the unpaired group during preconditioning (left) and recall (right) by bootstrap resampling in amygdala projecting dmPFC neurons. Angular distances in the paired group marked as red lines is within the 95% confidence intervals (CI) at preconditioning and recall. (k) Left: Median (of all rats in each group) Spearman correlation coefficient between calcium responses during auditory stimuli in recall and during foot-shocks of aversive conditioning for each rat in the paired and unpaired groups. Dots represent individual animal values (filled = male, open = female). Right: Similar to the left panel, but for the correlation coefficient between calcium responses during visual stimuli in recall and during foot-shocks of aversive conditioning. Mann-Whitney U test (two-sided); *: r = 74, p = 0.040; n.s.: r = 68, p = 0.19. (l) Pie-chart showing the percentage of shock-activated cells during aversive conditioning which were subsequently reactivated by auditory-only, visual-only or auditory and visual (Co, co-responsive) CSs at memory recall in the paired and unpaired groups. Chi-square Test (two-sided); *: X2 = 3.98, p = 0.046; n.s.(blue): X2 = 1.30, p = 0.25; n.s.(purple): X2 = 2.54, p = 0.11.
