Extended Data Fig. 8: Design of heterophilic protocadherin chimera pairs that lost homophilic binding.

a, Contact between cell membranes (grey) of astrocyte sister branches (WT). γC3 molecules (blue) forming a trans-dimer (in curly brackets) are shown schematically with extracellular cadherin (EC) domains as ellipses. b,c, Homophilic-deficient cPcdh chimeras. d, A pair of chimeras (one from b and another from c) co-expressed in the same astrocyte. e, A pair of chimeras in D modified with mutations (asterisk, cyan) that enable heterophilic binding. f, Summary of AUC experiments of WT γC3, γC4, and γC5, parts of which were used for chimera design. Of note, AUC on γC5 was done in the context of the EC1-EC5 fragment, but only the EC1-EC4 trans-dimer is shown in the schematic. g, Summary of AUC experiments on the designed chimeras. See Methods for details on mutation (cyan) design. A sign with a circle and a line through it depicts inability to form dimers.