Extended Data Fig. 1: Efficient labeling of cortical astrocytes using AAV.
a, Sparse labeling of astrocytes across all cortical layers (P14 mouse brain). AAV.PhP.eB expressing Lck-GFP, under the control of an astrocyte-specific promoter (GfaABC1D), which were retro-orbitally injected into neonates at P1. b, Astrocytes were labeled from P8 to P21 in the visual cortex, and tissues were subsequently stained with GFP antibodies. Representative images were obtained from three mice and have been routinely observed in injections with similar results.
