Extended Data Fig. 5: Inhibiting taurine synthesis in osteolineage cells impairs LSC growth.
From: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
a, b, Alizarin red staining (a), and Cdo1 and Csad expression (b) in MSCs undergoing osteogenic differentiation (mean ± s.d.; n = 3 technical replicates per cohort). c, CDO1 expression in murine leukaemia and patient AML MSCs undergoing osteogenic differentiation (mean ± s.e.m.; n = 3 replicates per time point). d, Taurine in MSC culture media during osteogenic differentiation (mean ± s.e.m.; n = 3 replicates; secreted over 48–72 h). e,f, Experimental strategy (e) and Cdo1 expression (f) in MSCs transduced with shRNAs targeting LacZ or Cdo1 (mean ± s.d.; n = 3 technical replicates per cohort). g, Live leukaemia cells (left), and CFU (right), post coculture with bcCML MSCs (mean ± s.e.m.; n = 7 independent culture wells per cohort (live); n = 6 (CFU); data combined from 2 independent experiments; one-way ANOVA). h, CDO1 expression in 293 T transduced with shRNAs targeting LACZ or CDO1 (mean ± s.d.; n = 3 technical replicates per cohort). i, Cdo1fl/fl model. j,k, PCR of Cdo1 (j) and Cdo1 expression (k) in Cdo1fl/fl and Cdo1fl/fl;Prrx1-Cre+ MSCs following osteogenic differentiation (mean ± s.d.; n = 3 technical replicates per cohort). l-n, BM stroma in leukaemic control and Cdo1fl/fl;Prrx1-Cre+ mice: MSCs (l), osteolineage cells (m), and, endothelial cells (n) (mean ± s.e.m.; n = 7 Cdo1fl/fl and n = 3 Cdo1fl/fl;Prrx1-Cre+; data combined from three independent experiments; unpaired two-tailed Student’s t-test). o-q, CFU of murine cKit+ AML cells (o), murine Lin− LSCs (p), or primary human AML cells (q) with taurine (mean ± s.e.m.; n = 3 independent culture wells from n = 2 murine AML, n = 2 murine bcCML, and n = 3 primary human AML samples; data combined from two independent experiments; one-way ANOVA). r, Impact of taurine supplements on murine bcCML progression in unirradiated recipients (n = 14 no taurine and 0.01 mg/ml taurine, n = 15 10 mg/ml taurine; data combined from three independent experiments; log-rank test). The mouse image in e is adapted from ref. 6, Springer Nature America.
