Extended Data Fig. 7: The role of TAUT in normal haematopoietic stem cell function.

a, Average number of BM cells in +/+ and −/− mice (mean ± s.e.m.; n = 5 animals per cohort). b-d Representative FACS plots, and quantification of frequency (left) and cell number (right) of hematopoietic stem cells (b, HSC: Lin⁻cKit⁺Sca⁺CD150⁺CD48⁻) and multipotent progenitors (b, MPPs: Lin⁻cKit⁺Sca⁺CD150⁻CD48⁻), committed granulocyte–macrophage progenitors (c, GMP: Lin⁻IL7Ra⁻Kit⁺Sca1⁻CD34⁺CD16/3⁺), common myeloid progenitors (c, CMP: Lin⁻IL7Ra⁻Kit⁺Sca1⁻CD34⁺CD16/32⁻), megakaryocyte–erythroid progenitors (c, MEP: Lin⁻IL7Ra⁻Kit⁺Sca1⁻CD34⁻CD16/32⁻), and differentiated haematopoietic cells (d) in the BM of +/+ and −/− mice (mean ± s.e.m.; n = 5 animals per cohort; data combined from four independent experiments). e, Complete blood count of indicated cells and haemoglobin content in the peripheral blood of age and sex-matched 8-week-old littermates (mean ± s.e.m.; n = 11 +/+ and n = 13 −/−; data combined from two independent experiments). f-g, Experimental strategy (f), donor engraftment in peripheral blood over time (g). h-k, Average donor chimerism (h), frequency of KLS (Lin⁻cKit⁺Sca⁺), HSCs, and MPPs (i), committed progenitors GMP, CMP, and MEP (j) and, differentiated haematopoietic cells (k) in primary recipient BM four months post-transplant (f-k: mean ± s.e.m.; n = 9 +/+ and n = 10 −/−; data combined from two independent experiments). l, Donor engraftment in peripheral blood of secondary recipients (mean ± s.e.m.; n = 6 animals per cohort; g, l, two-way ANOVA). m-q, Average donor chimerism (m), frequency of KLS, HSCs, and MPPs (n), committed progenitors GMP, CMP, and MEP (o), and differentiated haematopoietic cells (p, q) in BM of secondary recipients four months post HSC transplant (mean ± s.e.m.; n = 6 animals per cohort; a-e, h-k, m-q unpaired two-tailed Student’s t-test). The mouse images in f are adapted from ref. ⁶, Springer Nature America.

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