Extended Data Fig. 8: Taurine inhibition synergizes with venetoclax in vitro.
From: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
a, SLC6A6 expression in human AML stem cells, progenitors and mature blasts (Gene Expression Commons). b, SLC6A6 expression by karyotype or FAB subtype (TCGA-AML, n = 146 karyotype and n = 149 FAB subtype; dot represents an individual patient). c, SLC6A6 expression in relapse origin-committed (ROC) and relapse origin-primitive (ROP) AML34 (n = 29 ROC and n = 10 ROP); (b-c central line, box, and whiskers represent median, interquartile range, minimum/maximum point within 1.5*IQR respectively; unpaired two-samples Wilcoxon test). d, Taurine in Lin− bcCML cells treated with DMSO or TAG (mean±s.e.m.; n = 8 independent wells combined from 2 experiments). e, Taurine in Lin− bcCML cells treated with GES or water (mean±s.e.m.; n = 5 independent culture wells; d-e unpaired two tailed Student’s t-test). f-h, CFU of Lin− bcCML (f), ckit+ AML LSC (g) with DMSO or indicated doses of TAG, or Lin− bcCML with water or indicated doses of GES (h)(mean±s.e.m.; n = 3 independent culture wells per cohort; data combined from two independent experiments for f,h). i, j, Viability (i) and CFU (j) of murine Lin− bcCML cells treated with 0.5 mM venetoclax (ABT-199, Ven) for 48 h (n = 3 independent culture wells per cohort; data combined from two (i) or three (j) independent experiments; two-way ANOVA). k, CFU of Lin− bcCML cells with DMSO/water, Ven, or indicated doses of TAG (left) and GES (right), or indicated combinations (mean ± s.e.m.; n = 3 per independent culture wells; data combined from 2 or 3 independent experiments). l, CFU of cKit+ AML LSC with DMSO/water, Ven, GES, or indicated combinations (mean±s.e.m.; n = 3 independent culture wells; data combined from 2 independent experiments, f-h, j-l one-way ANOVA). m, Combination index of GES and Ven per fraction affected (left) and isobologram (right) by Chou-Talalay method (n = 3 independent culture wells; data combined from 3 independent experiments; colours represent sample type, shapes represent Ven/GES combinations).
