Extended Data Fig. 9: Taurine inhibition in vivo.
From: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
a, Survival curve showing the impact of treating mice transplanted with Lin− bcCML cells with GES (2.5%), Ven (50 mg/kg), or their combination (n = 10 control, n = 12 GES, n = 6 Ven, n = 8 GES/Ven); data combined from three independent experiments; lines below graph represent days of treatment; log-rank test). b, Taurine in 107 BM cells from leukaemic mice treated with indicated amounts of GES for 13–16 d (mean ± s.e.m.; n = 3 technical replicates per cohort). c, d, Experimental strategy (c) and mass (d) of mice during treatment with GES, TAG, or control (mean ± s.e.m.; n = 3 animals per cohort; two-way ANOVA). e,f, Viability (e) and average number of BM cells (f) (mean ± s.e.m.; n = 6 mice per cohort; data combined from two independent experiments). g-h, Representative FACS plots (left), and number (right) of HSC and MPP (g), and differentiated haematopoietic cells (h) (mean ± s.e.m.; n = 6 animals per cohort; data combined from two independent experiments; two-way ANOVA). i, Haematoxylin & Eosin staining of indicated tissues in treated animals. j, Intracellular taurine levels by LC/MS in BM cells from treated animals (mean ± s.e.m.; n = 8 independent replicates from n = 3 control, n = 10 from 4 GES, n = 9 from 3 TAG treated animals; each colour represents a mouse). k, l, SLC6A6 expression (k) and taurine levels (l) in K562 cells transduced with shRNAs targeting LacZ and SLC6A6 (mean ± s.d.; n = 3 technical replicates per cohort). m-o, CFU of human leukaemia cell lines transduced with lentiviral shRNAs targeting LacZ or SLC6A6, K562 (m), THP1 (n), and MV-4-11 (o) (mean ± s.d.; n = 3 independent culture wells per cohort). p, Relative SLC6A6 expression (left; mean ± s.d.; n = 3 technical replicates per cohort) and CFU (right) in MDS-L cells transduced with shRNAs targeting Luc and SLC6A6 (mean ± s.e.m.; n = 9 independent culture wells per cohort; data combined from 3 independent experiments; b, e-h, j, l-p one-way ANOVA). The mouse image in c is adapted from ref. 6, Springer Nature America.
