Extended Data Fig. 10: TAUT loss impairs energy metabolism in myeloid leukaemia.
From: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
a, Quantification of taurine and glycolysis associated metabolites in +/+ and −/− Lin− bcCML cells (mean ± s.e.m.; n = 16 samples from n = 6 +/+ leukaemic mice and n = 9 samples from n = 3 −/− leukaemic mice; unpaired two-tailed Student’s t-test with Welch’s correction). b, Curve and quantification of normalized of oxygen consumption rate (OCR) in Lin− bcCML cells (mean ± s.e.m.; n = 10 independent culture wells per cohort from 3 bcCML samples; data combined from three independent experiments; unpaired two-tailed Student’s t-test). c-e, Impact of supplementing 5 mM sod. acetate (c), lactate (d), or indicated amounts of glucagon (e) on the CFU of Lin− bcCML cells (mean ± s.e.m.; n = 3 independent culture wells per cohort; data combined from 2 independent experiments; one-way ANOVA). f, Experimental strategy used to determine 13C taurine tracing in K562 cells by untargeted metabolomics. g, Incorporation of 13C label from taurine in indicated metabolites (mean ± s.e.m.; n = 5 independent biological replicates). h,i, Colony-forming ability of Lin− bcCML cells in the presence of 160 mM N-acetyl taurine (h) or indicated amounts of glutaurine (i) (mean ± s.e.m.; n = 3 independent culture wells per cohort; data combined from two independent experiments; one-way ANOVA).
