Fig. 3: TAUT loss impairs myeloid leukaemia initiation and propagation in mouse models.
From: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
a, Relative Slc6a6 mRNA expression in whole bone marrow cells from Slc6a6+/+ (+/+) and Slc6a6−/− (−/−) mice. Data are mean ± s.d. n = 3 replicates per cohort. b, Taurine in normal bone marrow cells. Data are mean ± s.e.m. n = 6 pelvic bones from three animals per cohort; data were combined from two independent experiments. c,d, The experimental strategy (c) and primary bcCML survival curve (d). n = 12 (+/+) and n = 11 (−/−). Data were combined from three independent experiments. e, CFU analysis of Lin− bcCML cells from primary transplants. Data are mean ± s.d. n = 3 culture wells per cohort. f, Survival curve of secondary bcCML transplants. n = 11 (+/+) and n = 10 (−/−); data were combined from two independent experiments. g, CFU of Lin− cells from secondary transplants. Data are mean ± s.d. n = 3 independent culture wells per cohort. h, Taurine in Lin− LSCs. Data are mean ± s.e.m. n = 8 independent replicates per cohort from two independent samples. i,j, The experimental strategy (i) and survival curve (j) show the impact of TAUT loss on de novo MLL-AF9-driven AML. n = 17 (+/+) and n = 20 (−/−); data were combined from four independent experiments. k, Taurine in KIT+ AML cells. Data are mean ± s.e.m. n = 4 animals per cohort. l, CFU analysis of KIT+ AML cells. Data are mean ± s.d. n = 3 culture wells per cohort. m,n, Experimental strategy (m) and survival curve (n), showing the impact of TAUT loss on de novo AML-ETO9a-driven AML. n = 7 (+/+) and n = 10 (−/−). Data were combined from two independent experiments. o, Representative FACS plots and quantification of the Lin−CD150−SCA1+FLT3+ bcCML stem cell frequency in the bone marrow (left) and spleen (right) of recipients. Data are mean ± s.e.m. n = 11 animals per cohort. Data were combined from three independent experiments. p, Representative FACS plots and quantification of early apoptosis and necrosis in bcCML at 14 days after transplant. Data are mean ± s.e.m. n = 8 animals per cohort. Data were combined from two independent experiments. q, Representative FACS plots and graph showing the frequency of in vivo BrdU incorporation in bcCML. Data are mean ± s.e.m. n = 3 animals per cohort. Statistical analysis was performed using unpaired two-tailed Student’s t-tests (b, e, g, h, k, l and o–q) and log-rank tests (d, f, j and n). The mouse images in c, i and m are adapted from ref. 6, Springer Nature America.
