Extended Data Fig. 11: Analysis of factors contributing to MRRWMR and KRHWMR PAM preferences.
From: Custom CRISPR–Cas9 PAM variants via scalable engineering and machine learning
a, Structural prediction of an alternative conformation of the S1136R mutation leading to additional hydrogen bonding with T at position 3 of the PAM. b–e, SHapely Additive exPlanations48 (SHAP) values for PAMmla predictions for MRRWMR (panels b,c) and KRHWMR (panels d,e) interacting with NGTG (panels b,d) or NGGG PAMs (panels c,e) PAMs. Feature values are shown in gray (1: mutation is present, 0: mutation is absent). Red represents features with positive impact on predicted rate constant and blue represent features with negative impact on predicted rate constant.
