Extended Data Fig. 2: Geographic distribution of mutation burden.

Box plots indicating the distribution of single base substitutions (SBS), small insertions and deletions (ID), doublet base substitutions (DBS), copy number alterations (CN), and structural variants (SV) across countries for microsatellite stable (MSS) colorectal tumors. Box plots and data points representing total number of mutations for each variant type were colored according to each country’s colorectal cancer age-standardized incidence rates (ASR) per 100,000 individuals. A horizontal blue line indicates the median mutation burden for each variant type. Statistically significant differences were evaluated using multivariable linear regression models comparing each country to all others and adjusted by age of diagnosis, sex, tumor subsite, and tumor purity. P-values were adjusted for multiple comparisons using the Benjamini-Hochberg method based on the total number of countries assessed and reported as q-values. The line within the box indicates the median, while the upper and lower ends indicate the 25^th and 75^th percentiles. Whiskers show 1.5 × interquartile range, and values outside are shown as individual data points.