Extended Data Fig. 5: Spatial characterization of CMs.
From: Cross-tissue multicellular coordination and its rewiring in cancer
(a,b) Spatial distribution of CM08 and CM10 (a) and their component cell subsets (b) in a Visium section of the skin. (c) Spatial aggregation (based on Moran’s I) among cell subsets within CM08 or CM10 in the Visium section shown in a,b. (d) Averaged spatial aggregation (based on Moran’s I) among cell subsets within CM08 or CM10 across six Visium sections from six available donors. (e–g) H&E staining (e), spatial distribution of CMs (CM02, CM03, and CM05) (f), and cellular niches identified by CellCharter (g) in three adjacent Visium sections of the ileum from one donor. (h) CODEX imaging of intestinal Peyer’s patches showing B cells (CD21, green), T cells (CD3, pink), and DCs (CD11c, cyan). Scale bars, 200 μm. Data from the HuBMAP portal (dataset ID: HBM785.FJVT.469). (i) Dot plot illustrating scRNA-seq expression of a gene panel differentiating CM02 and CM03 among various cell subsets in the colon. The percentages of colored subsets among similar subsets are indicated at the top. For example, 98.2% of the plasma cells (B10, B11, and B12) are B12.
