Fig. 4: Multicellular dynamics in context.
From: Cross-tissue multicellular coordination and its rewiring in cancer
a, CM05 and CM06 activities in spleen samples across age groups. Dots represent individual samples. <35, n = 3; 40–49, n = 8; 50–59, n = 10; 60–69, n = 7; 70–85, n = 1. Kruskal–Wallis tests and two-tailed unpaired Wilcoxon tests. b, Sample-averaged frequencies of cell subsets across age groups. c, Circular heat map (left) showing cellular activity of 17 convergent regulons in CM05 (right) across age groups. Min, minimum; max, maximum. d, Sample-averaged expression of CM12 MCP genes (rows) across breast samples (columns). Genes are categorized by their direction (up- or down-regulation, left colour bar) and associated cell subsets (right colour bar). Samples are sorted by overall expression (top bar plot) and are labelled by menopausal status (top colour bar). e, Distribution of CM12 MCP overall expression in pre- and post-menopausal breast cells grouped by cell subsets. Vertical black line indicates the median of the distribution. Two-tailed unpaired Wilcoxon tests. f, CM12 activity (left) and frequencies of fibroblast subsets S10 (middle) and S06 (right) in pre-menopausal (n = 93) and post-menopausal (n = 18) samples. Two-tailed unpaired Wilcoxon tests. g, Visium analysis of the breast showing histopathological regions annotated (left) and spatial distribution of fibroblast subsets S10 (middle) and S06 (right). h, PHATE (potential of heat diffusion for affinity-based trajectory embedding) visualization of breast samples (dots) coloured by menopausal status (left), pseudotime (middle) or CM12 activity (right) (Methods). i, Heat map showing frequencies of CM12 subsets (rows) across breast samples sorted by pseudotime. j, Trend lines show LOESS-smoothed fibroblast inflammatory scores along pseudotime in breast samples. The error bands show the 95% confidence intervals. For box plots in a,f, the centre line represents the median, the box limits delineate the top and bottom quartiles, and the whiskers extend to the highest and lowest values within 1.5× the interquartile range.
