Fig. 1: Multilocus microsatellite typing reveals two clades of P. destructans.
From: Two distinct host-specialized fungal species cause white-nose disease in bats
a, Sampling locations in Eurasia (North American sites not shown; n = 255). b, Phylogenetic tree, based on the DA distance, representing the relationship between all 1,866 unique multilocus genotypes (North American isolates are indicated with an asterisk) originating from the 5,479 isolates from 264 sites based on 18 microsatellite loci (for the DA distance, see the section ‘Analyses of multilocus genotypes’ in the Methods; the monophyly of Pd-1 and Pd-2 was consistently recovered when jackknifing loci). For better visualization of both clades, the section containing Pd-2 was magnified. c, Principal component analysis (bottom) of isolates (Pd-1 was subsampled to ensure even sampling between clades and to maximize geographical coverage, which resulted in 234 isolates for Pd-1 and 92 isolates of Pd-2; Supplementary Table 1). Density (top) of principal component 1 (PC1) coefficients after random subsampling of Pd-1 to obtain the same number of sampling sites as for Pd-2 (51 sites, replicated 100,000 times). Dotted lines represent the absolute edges of the distribution (see the section ‘Analyses of multilocus genotypes’ in the Methods for details).
