Extended Data Fig. 4: Induction of maternal–fetal anaemia by the iron chelator DFX.
From: Maternal iron deficiency causes male-to-female sex reversal in mouse embryos
a, Immunoblot analysis of H/L-ferritin in E11.5 XY NR5A1+ cells in embryos carried by DFX-treated mothers and those carried by control mothers. GAPDH is used as an internal control. b, Effect of treating pregnant mothers with DFX an the number of gonadal somatic cells in developing embryos in utero. NR5A1+ cells were isolated from E11.5 XY embryos carried by DFX-treated mothers and those carried by control mothers. Numbers of embryos examined are shown above the bars. Data are presented as mean ± s.d. c, Expression profiles of Sry, Nr5a1, Gata4, Wt1, Kdm3a, Tfrc, Hmox1, Myl7, and Apoc1 in E11.5 XY NR5A1+ cells in the embryos carried by DFX-treated mothers and those carried by control mothers. NR5A1+ cells were purified from E11.5 embryos and subjected to RT–qPCR analysis. Numbers of embryos examined are shown above the bars. Data are presented as mean ± s.d. All differences between means with P < 0.01 are indicated; two-tailed paired t-tests.
