Extended Data Fig. 5: Cryo-EM data processing workflow for raiA motif. | Nature

Extended Data Fig. 5: Cryo-EM data processing workflow for raiA motif.

From: Naturally ornate RNA-only complexes revealed by cryo-EM

Extended Data Fig. 5

(a) Data processing flowchart. (b) Representative micrograph (10,825 micrographs total) and 2D class averages. (c) Fourier shell correlation (FSC) plot. (d) Plot of particle number against the reciprocal squared resolution. The B-factor was calculated as twice the linearly fitted slope74. (e) Local resolution on the cryo-EM map (top) and the molecular model (bottom). (f) Resolvability of the built model as measured by Q-score. The expected Q-score of a RNA model at this resolution is labeled with a blue dotted line.

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