Extended Data Fig. 7: Binding of KIR2DL1-binding RIFINs to KIR2DS1 and modulation of KIR2DS1 activity.
From: RIFINs displayed on malaria-infected erythrocytes bind KIR2DL1 and KIR2DS1
a) SPR analysis of the binding of different KIR2DL1-binding RIFINs to immobilised KIR2DS1. In each case, a concentration series was conducted from 40 µM (KEN-02, -04, SEN-01, -08). or 20 µM (KEN-01, -06, SEN-12). Affinities calculated by equilibrium fitting from at least 3 independent experiments (PRISM10) are denoted within each box. b) Effect of RBK21 on CD107a expression and production of IFN-γ and TNF-α production in the gated KIR2DS1-(+) subset were assessed for a different donor to that tested in Fig. 4. The 3D7 strain and iRBC expressing PF1254200 (ctl-RIFIN) were used as negative controls. Data represent the mean (n = 9 independent measurements from one donor) with **** P < 0.0001 (two-sided Student’s t-test). Exact P values in source data. c) The structure of KIR2DL1 bound to HLA-Cw4 (PDB 1IM9) was overlaid with a homology model (SWISS-MODEL) of KIR2DS1. Interfacial residues are shown as sticks for KIR2DL1 (green) and KIR2DS2 (blue). The 91Thr/Lys mutation at this interface is labelled. Also shown is a multiple sequence alignment of the regions responsible for the interaction, with residues involved in interactions highlighted in orange (above inset).
