Extended Data Fig. 9: GRP and ERα modulate melanoma cell invasion and in a sex- and E-cadherin–dependent manner.
From: Targeting GRPR for sex hormone-dependent cancer after loss of E-cadherin
a, Invasion assay of 1057 mouse melanoma cells, quantified by counting DAPI-stained nuclei that migrated through the Matrigel layer. Scale bar = 500 µm. b-e, Quantification of invading cells in various melanoma cell lines: male Ecad 1181 (b), female Ecad 1039 (c), male ΔEcad 1456 (d), and female ΔEcad 1057 (e) treated with 10 nM GRP, 1 μM RC-3095, and/or 1 μM ICI. f, Western blot analysis of ERα protein levels in mouse melanoma cells treated or not with ICI (1 μM, 24 h) with actin as loading control. g, Histograms showing ERα quantification from three independent biological experiments. GRP-induction significance was assessed by two-sided Mann-Whitney adjusted with a Benjamini–Hochberg test. Quantification was evaluated by ANOVA on the log transformed data. Data are represented as mean ± SD. ≥ 3 independent biological replicates were performed per experiment.
