Extended Data Fig. 3: Hepatocyte molecular subtypes across human, lemur, and mouse.
From: A molecular cell atlas of mouse lemur, an emerging model primate
a. UMAP of liver hepatocytes and cholangiocytes, separately for human (left), lemur (middle), and mouse (right). Top to bottom: cells colored by cell type annotation, by sex of the animal, and by heatmap showing relative expression of a hepatocyte marker (ASGR1), a cholangiocyte marker (SPP1), and a hepatocyte subtype DEG (CPN2). Note that in the lemur atlas CPN2hi and CPN2low hepatocytes are given the designations hepatocyte (APOB+) and hepatocyte (PHYH+), respectively. b. Species-integrated UMAP of liver hepatocytes and cholangiocytes, with cells colored by cell type annotation (top) and species (bottom). c. Box and whisker plots of the number of genes (top) and UMIs (bottom) detected per cell for each cell type indicated. H, Human; L, lemur; M, mouse. d. Dot plot comparing mean expression (ln(UP10K+1), dot heamap) and percent of cells (dot size) expressing indicated genes in the two hepatocyte molecular subtypes and cholangiocytes across human, lemur and mouse. Genes shown are (top to bottom): one-to-one orthologues of hepatocyte and cholangiocyte markers, DEGs between the two hepatocyte subtypes, liver zonation markers27, and cell stress markers including immediate-early genes and heat shock proteins (labeled with the respective human gene symbol). Note that in all three species, hepatocyte molecular subtypes do not differ significantly in expression of zonation markers or cell stress markers.
