Extended Data Fig. 5: Screening for potential cross-reactive candidate epitopes capable of stimulating naïve TCR7B8 CD4 T cells in vitro.
From: Gut inflammation promotes microbiota-specific CD4 T cell-mediated neuroinflammation
(A) A BLAST search was used to identify a list of candidate cross-reactive peptides. Core epitopes for TCR7B8 and matched amino acids in candidate peptides are highlighted in red. Amino acids that did not match core epitopes for TCR7B8 are shown in blue. (B) The percentage of TCR7B8 CD4 T cell activation in response to stimulation with a 100 μM concentration of candidate peptides. T cell activation was determined based upon CD25 and CD69 expression at 24 h following co-culture. (C) The dose-dependency of candidate peptide-induced T cell activation at 24 h following co-culture. In total, 11 candidates selected based on the results in (B) were further tested for their ability to activate TCR7B8 CD4 T cells at concentrations of 10 μM and 0.4 μM. The results shown in (B) were also shown on the same graph in (C). Screening analyses were performed one time. P2X6 (ID3), ANAPC2 (ID4), ErbB2 (ID5), IFT46 (ID6), and TRO (ID11) were further generated as purified peptides and tested in Fig. 3.
