Extended Data Fig. 7: Contributions of Foxp3+ regulatory T cells in TCR7B8-mediated CNS inflammation.
From: Gut inflammation promotes microbiota-specific CD4 T cell-mediated neuroinflammation
(A) Schematic representation of Treg depletion in SFB TCRTg Foxp3 DTR mice. Adult (8–14 weeks old) TCRTg+ or non TCRTg with or without Foxp3 DTR mice were i.p. injected with 10 μg DT every other day, 4-times. (B) Neurological scores of indicated mouse groups. (C) Efficacy of Treg depletion. Frequencies of Foxp3+ Tregs among CD4 T cells in the colon of indicated mice at Day 8 (left: 7B8 Foxp3 +/+ DT (n = 3), 7B8 Foxp3DTR +DT (n = 4), No TCR Tg Foxp3DTR + DT (n = 3)) and Day 14 (right: 7B8 Foxp3+/+ + DT (n = 4), 7B8 Foxp3DTR +DT (n = 10), No TCR Tg Foxp3DTR + DT (n = 3)), 1A2 Foxp3DTR +DT (n = 5)) following the first DT injection. (D) Frequencies of CD4 T cells (left) and IFNγ/IL-17A double producing CD4 T cells (right) in the brains of indicated mouse groups. (E) Schematic representation of TCR7B8 or TCR1A2 naïve CD4 T cells transfer into the Treg depleted Foxp3 DTR mice carrying polyclonal CD4 T cells. The sorted naïve CD4 T cells (200,000 cells) collected from TCR7B8 or TCR1A2 mice were R.O. transferred Treg depleted Foxp3DTR mice followed by DT injection. (F) Neurological scores of indicated mouse groups. Results are presented as the mean ± SEM, with dots representing individual mice. (B) Two-way ANOVA, (C, D) One-way ANOVA, (F) Two-Tailed-Student’s t-test.
