Extended Data Fig. 10: Analysis of thymopoiesis in cartilaginous fishes.
From: Developmental trajectory and evolutionary origin of thymic mimetic cells
a, Expressed genes in the thymus of the brown-banded bamboo shark (C. punctatum); minus-RT control reactions (–) are shown in parallel; size markers are given in base pairs (bp). For gel source data, see Supplementary Fig. 8. b, Characterization of the thymus in S. canicula. Micrographs of the thymus after RNA in situ hybridization with the indicated probes. Cells expressing the indicated genes turn blue. c, Micrograph of the thymus region. Haematoxylin-eosin staining of histological section is shown on the left; micrographs of the thymus after RNA in situ hybridization with the indicated probes are shown in the other panels. d, Thymus tissue section stained with alcian blue to identify mucus-producing cells, highlighted in the inset. For a-d, results are representative of n = 2 animals with similar results. e, Signature enrichment analysis of mouse Foxn1−/− mutants expressing the C. milii Foxn1 and Foxn4 genes under the control of the mouse Foxn1 promotor compared against corresponding wildtype controls. Log transformed p values of upregulated sets were multiplied with −1 so that positive values indicate upwards directionality, and negative values indicate downwards directionality, respectively. Scale bars in b, c represent 0.2 mm; for d, 0.05 mm.
