Fig. 3: FOXN1 influences the development of mimetic cells.
From: Developmental trajectory and evolutionary origin of thymic mimetic cells
a, Ratio of Ly51+ (cTEC) and UEA1+ (mTEC) EPCAM+CD45− TECs cells over developmental time. Each point shows the ratio from one thymus. The trend line was determined via LOESS with tenfold cross-validation. The minimum occurs at P21. Error bands show 95% confidence interval around the predicted trend line. b, Multiple sequence alignment of the C-terminal end of FOXN1 protein sequences encoded by exon 2 of the gene in various jawed vertebrate species; identical amino acid residues are shaded. Cm, Callorhinchus milii; Dr, Danio rerio; Xl, Xenopus laevis; Gg, Gallus gallus; Oa, Ornithorhynchus anatinus; Mm, Mus musculus. Sequences obtained from ref. 38. c, Schematic illustrating the coding content of Foxn1 exons and the deleted 3′ region of Foxn1 coding exon 2 in the Δ3ex2 mutant; three exons contribute to the DNA-binding domain as indicated. d, Principal component analysis of bulk RNA-seq samples from purified TECs of Δ3ex2 mutants, collected from mice around P21. e, Numbers of CD4/CD8-double positive thymocytes in thymi of wild-type FVB mice and Δ3ex2 mutants during collapse and recovery phases. n is indicated; each data point is one thymus explant from one animal. P values between groups were derived from pairwise two-sided t-tests with Bonferroni correction after significant (P < 0.05) ANOVA result. Boxes encapsulate the first to third quartile, the line indicates the median and whiskers extend to the furthest point with a distance of up to 1.5 times the interquartile range from the boxes. f, Signature enrichment analysis in bulk RNA-seq data of purified TECs in Δ3ex2 mice (FVB (wild type), n = 6; collapse, n = 9; recovery, n = 15). P values from camera (two-sided, Benjamini–Hochberg adjusted) were log-transformed and multiplied by −1 for upregulated sets, so that positive values indicate upwards directionality and negative values indicate downwards directionality. Values beyond the limits of the colour scale were rounded to the nearest limit. Adj., adjusted.
