Extended Data Fig. 2: Expression patterns of TEC-specific genes in the mouse thymus.
From: Developmental trajectory and evolutionary origin of thymic mimetic cells
a, Micrographs of P0 thymus sections hybridized with the indicated probes. b, c, Higher magnification views of the Myog hybridization pattern at P0 (b) and P28 (c). d, Enumeration of Myog-positive cells in thymus sections of P0 (n = 6) and P28 (n = 7) mice. Each data point represents a different thymus section. Data are shown as mean ± SD; p = 1.5 × 10−6, two-sided t test. Results in a-d are representative of n = 2 mice of each time point with similar results. e, RNA in situ hybridization of consecutive sections of Foxn1+/+ thymi at P28, hybridized with probes specific for Foxn1 (pan TEC marker) and Hnf4a, a marker of the enterohepatic mimetic lineage. The relevant tissue compartments are indicated (sc, subcapsular region; c, cortex; m, medulla). Note that the Hnf4a signal is confined to the medullary region. f, Higher magnification views of the medullary regions of P28 thymi hybridized with the indicated probes. Results in e, f are representative of n = 3 mice with similar results. Scale bars: a, 0.2 mm; b, c, e, f, 0.1 mm.
