Extended Data Fig. 9: Multiplexed immunohistochemistry on matrix perturbations.
From: Morphodynamics of human early brain organoid development
a) Selected images show stainings of ECM proteins on day 15 and 21 on organoid slices in Matrigel and no-matrix conditions. b) Images show cross sections from an immunohistochemistry experiment on organoids (day 15) grown with Matrigel (n = 4) or with no-matrix (n = 4) and stained to show nuclei (DAPI), COL4A1 and CDH2. Scale Bar is 100 micrometers in all images. c) Example organoids from each condition (Matrigel, no-matrix) on day 15 and day 21 colored by cell clusters projected back to the image. Boxes highlight the luminal region from insets are shown in d. d) Images show lumen in organoids shown in c with ARL13B and LAMA1 protein expression marking neuroepithelium polarity. e,f) Images show protein stainings on organoid slices that were embedded in 0.6% agarose, fixed and stained in a 4i experiment. Day 7 (n = 2) and day 11 (n = 3). Images show polarity marker proteins (e) and ECM proteins (f). Scale, 100 micrometers in all images.
