Extended Data Fig. 2: Detection of lentivirus transduction and rabies virus infection in human organotypic cultures.

(a) Quantification of GFP⁺ staining (indicating transduction by the helper lentivirus that delivers TVA and G) as determined by brightfield immunofluorescence detection (each dot represents a separate culture, and n = 6 organotypic cultures per condition from epilepsy surgery (non-GBM)). (b) Quantification of GFP⁺ cells by cell type. Each slide was co-stained for GFP, DAPI, and one cell type marker. Each dot represents a separate culture, and n = 5 organotypic cultures per condition from epilepsy surgery. (c) Quantification of apoptotic GFP⁺ cells by cell type using cleaved caspase-3 as a marker of apoptosis. Each slide was co-stained for GFP, one cell type marker, and cleaved caspase 3. Each dot represents a separate culture, and n = 5 organotypic cultures per condition from epilepsy surgery (non-GBM). (d) Quantification of mCherry⁺ staining (indicating rabies infection) as determined by brightfield immunofluorescence detection. Each dot represents a separate culture, and n = 6 organotypic cultures per condition. (e) Quantification of mCherry⁺ cells by cell type. Each slide was co-stained for mCherry, DAPI, and one cell type marker. Each dot represents a separate culture, and n = 5 organotypic cultures for astrocytes, n = 3 cultures for other cell types. (f) Quantification of apoptotic mCherry⁺ cells by cell type using cleaved caspase 3 as a marker of apoptosis. Each slide was co-stained for DAPI, mCherry, one cell type marker, and cleaved caspase 3. Each dot represents a separate culture, and N = 5 organotypic cultures for astrocytes and n = 3 for other cell types. (g-p) Representative images of control and lentivirus transduced cultures. (q-z) Representative images of control and rabies virus-infected cultures. Data are mean and error bars show ± SEM (a-f).

Source Data