Extended Data Fig. 1: B. bacteriovorus lifecycle, interaction with ECOR14, and details for plaque assays.
From: Functional amyloid proteins confer defence against predatory bacteria
(a) Model depicting the B. bacteriovorus predatory lifecycle. (b) Graphical representation of how modified double-agar overlay infection assays are performed. Briefly, prey and B. bacteriovorus strains are prepared separately. The prey is then mixed with serial dilutions of B. bacteriovorus and the mixture is embedded in soft agar (0.35 % agar) that is overlayed in 6-well dishes containing 1.6% agar underlays. (c) Efficiency of plating, calculated as PFU/mL, of B. bacteriovorus HD100 when infecting the indicated strain of E. coli or K. pneumoniae. Experiments were conducted as depicted in (b). Data are graphed as in Fig. 1b. (d) Efficiency of plating, calculated as PFU/mL, of B. bacteriovorus 109J when infecting the indicated strain of E. coli or K. pneumoniae. Experiments were conducted as depicted in (b). Data are graphed as in Fig. 1b. For c-d; two-sided Student’s t-test. *P < 0.05, **P < 0.001, *** P < 0.0001; precise P-values can be found in Source Data Table 5.
