Extended Data Fig. 2: ECOR14 defense against B. bacteriovorus in liquid culture.
From: Functional amyloid proteins confer defence against predatory bacteria
(a) Graphical representation of how liquid-based infection assays are performed. Briefly, prey and B. bacteriovorus strains are prepared separately. The prey and B. bacteriovorus are then mixed in a liquid culture and at various times post infection prey cell colony formation was quantified. The initial ratio of B. bacterivorous plaque forming units to E. coli colony forming units (multiplicity of infection, MOI) used in these assays was ~ 0.01. (b–c) Quantification of colony forming units (CFU) for the indicated strain of prey bacteria. Prey were either uninfected or infected with B. bacteriovorus (b) HD100 or (c) 109J in a liquid culture as depicted in (a). Data are the mean ± S.E.M. for n = 3 biological replicates.
