Extended Data Fig. 7: Lowering ethylene and hypoxia signalling rescue periderm regeneration under submergence conditions.
From: Plants monitor the integrity of their barrier by sensing gas diffusion
a-c, Cross sections of 18-day-old proPER15:erVenus roots in wild-type (a), ein2-1 (b), or etr1-3 (c) background at 1 dai. After the injury, the seedlings were grown on MS plates in ambient air (Ambient air, left), submerged in aerated liquid MS media (Aerated liquid MS), or oxygenated liquid MS media (Oxygenated liquid MS) for one day. d-f, The proportion of cells at the wound site showing Venus signal intensities above the threshold was quantified at 1 dai in 18-day-old proPER15:erVenus roots grown on MS plates (Ambient air), submerged in aerated liquid MS media (Aerated) or in oxygenated liquid MS media (Oxygenated). g-i, Cross sections of 19-day-old wild-type (g), ein2-1 (h), or etr1-3 (i) roots at 4 dai. After the injury, the seedlings were submerged in liquid MS medium without gas supplementation (Liquid MS, left), in aerated liquid MS media (Aerated liquid MS, middle) or oxygenated liquid MS media (Oxygenated liquid MS, right) for four days. j, The density of suberized cells at the wound site was quantified in 19-day-old wild-type, ein2-1, and etr1-3 roots at 4 dai grown in liquid MS medium without supplementing gas (Liquid), aerated liquid MS media (Aerated), or in oxygenated liquid MS media (Oxygenated) for four days. Kruskal-Wallis test followed by Dwass-Steel-Critchlow-Fligner pairwise comparisons was used (different characters indicate statistically significant differences between two groups; P < 0.05). n indicates the number of examined cross sections. Venus signal intensities in a-c or intensity of suberin staining with Fluorol yellow in g-i were shown according to the colour map on the right. White, SR2200 (cell wall). Scale bars: 50 µm.
