Extended Data Fig. 8: Ethylene and hypoxia signalling regain the pre-injury levels after periderm re-establishment.
From: Plants monitor the integrity of their barrier by sensing gas diffusion
a,b, Cross sections of 20-day-old RPS5A:erVenus (a) and RPS5A:erVenus-EBF1UTR (b) roots at 0 dai (left), 2 dai (middle) and 5 dai (right). RPS5A:erVenus signals were increased in the region beneath the regenerated periderm at 5 dai, but otherwise its expression level was not affected in the phloem parenchyma at 2 and 5 dai compared to the control. Therefore, RPS5A:erVenus-EBF1UTR signal intensities were examined in the phloem parenchyma neat the wound except for the area beneath the re-established periderm. c,d, Cross sections of 21-day-old proPCO1:erVenus and proPCO2:erVenus roots at 0 dai (left), 2 dai (middle) and 4 dai (right). e,f, Quantification of RPS5A:erVenus, RPS5A:erVenus-EBF1UTR signals in distal phloem parenchyma at 0, 2, 5 dai (e), and proPCO1:erVenus and proPCO2:erVenus signals in the vascular region at 0, 2, and 4 dai (f). Kruskal-Wallis test followed by two-sided Mann-Whitney U-test with the Bonferroni adjustment was used to test significant difference (ns; P ≥ 0.05, **; P < 0.01). Venus signal intensities are shown according to the colour map on the right. White, SR2200 (cell wall). Scale bars: 50 µm.
