Extended Data Fig. 4: Ethylene reporter expression after ACC treatment and ethylene emission upon wounding.
From: Plants monitor the integrity of their barrier by sensing gas diffusion
a, Cross sections of 18-day-old RPS5A:erVenus and RPS5A:erVenus-EBF1UTR roots grown on MS plate supplemented without (Mock), or with 2 or 10 µM ACC for one day after the injury. Venus signal intensities were measured in the vascular cambium. RPS5A:erVenus-EBF1UTR signals showed a gradual decline as the ACC concentration increased, confirming that ACC treatment increases ethylene signalling level in wounded roots. b, Cross sections of 17-day-old RPS5A:erVenus and RPS5A:erVenus-EBF1UTR roots without injury (Intact) and 8 or 11 h after injury. Venus signal intensities were measured in the distal phloem parenchyma of intact tissues or distal phloem parenchyma near the wound. c, Quantification of concentration of ethylene emitted from roots. The mature part of the wild-type roots was intact (Control) or longitudinally injured (Wounded). The ethylene concentration was measured after 3, 6, and 24 h from the injury. Higher ethylene concentration was always detected from wounded roots at all time points in each replicate. Kruskal-Wallis test (P < 0.05) followed by Pairwise Two-tailed Welch’s t-test for multiple comparisons with holm correction was used for a,b (different characters indicate statistically significant differences between two groups; P < 0.05). Two-tailed Welch’s t-test was used for c (ns; P ≥ 0.05, *; P < 0.05). n indicates the number of examined cross sections in a,b or the number of repeats in c. White boxes in a mark empty corners of stitched images. Venus signal intensities are shown according to the colour map on the right. White, SR2200 (cell wall). Scale bars: 50 µm.
