Extended Data Fig. 7: Myenteric CGRP+ neurons in colchicine-treated tissues show calbindin immunoreactivity.
From: Nutrients activate distinct patterns of small-intestinal enteric neurons
a–c, Confocal images of a colchicine-treated LMMP preparation immunolabelled for a, calcitonin gene-related peptide (CGRP) (cyan), b, calbindin (magenta), and c, Hu (pan-neuronal marker; green). d,e, Merged images illustrating the overlap between CGRP-, calbindin- and/or Hu-immunoreactivity. Scale bars, 50 µm. f, Graph showing the percentage of total Hu+ myenteric neurons that are CGRP+ and the percentage of total Hu+ myenteric neurons that are both CGRP+ and calbindin+ (n = 10 FOVs assessed; n = 458 Hu+ neurons, N = 2 mice). Most CGRP-immunoreactive neuronal cell bodies also show calbindin-immunoreactivity. Data are mean ± s.e.m.
