Extended Data Fig. 8: Timing of myenteric and submucosal responses to mucosal high K+.
From: Nutrients activate distinct patterns of small-intestinal enteric neurons
a–a”, Representative spinning disk confocal microscope recording of Ca2+ activity in the MP and SMP in response to mucosal high K+ application (from t = 20 s to t = 30 s). Maximum XY projections of the Z stack (25 µm) divided into the (a) MP and (a’) SMP. a”, Maximum XY and XZ projections of the full stack with the MP and SMP depth colour-coded in green and magenta, respectively. Scale bars, 50 µm. Responding neurons are indicated by colour-coded arrowheads. b, Corresponding traces of Ca2+ responses in the myenteric (green) and submucosal neurons (magenta) indicated in a–a”, depicted as a change in fluorescence over time. Arrowheads mark the Ca2+ peak of the initial responders. c, The Ca2+ peak of the first myenteric neuron that responded consistently preceded that of the first submucosal neuron that responded in corresponding preparations (n = 3). d, The percentage of myenteric and submucosal neurons that responded to mucosal high K+ (n = 3 preparations). Data are mean ± s.e.m.
