Extended Data Fig. 9: Submucosal responses to nutrients perfused onto the mucosa in peeled preparations.
From: Nutrients activate distinct patterns of small-intestinal enteric neurons
a–c”, Nutrient responses in peeled mucosa–SMP preparations. a,a’, Mucosal perfusion of glucose (300 mM) activates a subset of submucosal neurons as marked by white arrowheads. Glucose was applied from t = 10 s to t = 40 s. a”, Corresponding Ca2+ transients in the responding neurons indicated in the previous panels. b–b”, Mucosal perfusion of acetate (100 mM) activates a subset of submucosal neurons as marked by white arrowheads. Acetate was applied from t = 10 s to t = 40 s. c–c”, Mucosal perfusion of l-Phe (100 mM) activates a subset of submucosal neurons as marked by white arrowheads. Acetate was applied from t = 10 s to t = 40 s. Scale bars, 20 µm. d–f, Nutrient responders in the SMP of full-thickness (FT) preparations versus peeled preparations with the MP removed. While the percentage of submucosal responders to d, glucose were comparable (peeled: n = 8 FOVs examined; FT: n = 5 FOVs), fewer responders were observed in response to e, acetate and f, l-Phe in peeled preparations compared to in FT preparations (two-sided unpaired t-test; acetate, peeled: n = 15 FOVs, FT: n = 7 FOVs; l-Phe, peeled: n = 18 FOVs, FT: n = 10 FOVs). Data are mean ± s.e.m. Drawing in a is adapted from ref. 30 (Wiley).
