Extended Data Fig. 1: Many nutrient-responsive myenteric neurons express Nmu.
From: Nutrients activate distinct patterns of small-intestinal enteric neurons
a–r, Myenteric (GCaMP+GFP+) neurons which responded to the mucosal application of glucose (300 mM) (n = 2 ganglia, N = 2 mice) (a–f), acetate (100 mM) (n = 4 ganglia; N = 3 mice) (g–l) and l-Phe (100 mM) (n = 6 ganglia; N = 4 mice) (m–r) together with the corresponding RNAscope labelling for Nmu and Cck. Tissues were immunostained with anti-GFP to enhance the GCaMP signal after RNAscope and nuclei were counterstained with DAPI. Scale bars, 20 µm. Ca2+ transients in the nutrient-responsive neurons (arrowheads) are also shown (a’,g’,m’). Nutrient solutions were applied from t = 10 s to t = 40 s.
