Extended Data Fig. 2: Myenteric responses to sequentially applied nutrients.

a–d, Sequential application of glucose (300 mM) (a,a’), acetate (100 mM) (b,b’) and l-Phe (100 mM) (c,c’) by mucosal perfusion activates common myenteric neurons as well as neurons that respond uniquely to one nutrient—many neurons responded only to acetate (d). n = 706 neurons, N = 24 preparations examined (13 mice). The specific sequence of nutrient application was alternated between experiments. Each nutrient application was separated by 10 min of Krebs perfusion. Nutrient-responsive neurons are marked by arrowheads. Corresponding Ca²⁺ transients in responding neurons are shown in a’–c’, Scale bars, 20 µm. Drawing in a is adapted from ref. ³⁰ (Wiley).