Extended Data Fig. 3: Proteomic analysis of the size-exclusion chromatography step of fractionation of the Elp-Hdr-Fmd complex.
From: Electron flow in hydrogenotrophic methanogens under nickel limitation
a-d. Mass spectrometry-based proteomic analysis of the size-exclusion chromatography step of fractionation of the Elp-Hdr-Fmd complex shown in panel Fig. 2f. HdrABC (a), ElpABC (b), FwdA, FmdB, and FmdC (c), and MvhAGDB (d). The total number of identified peptide spectra matches for the protein (PSM) (n = 1) is shown as the intensity of the proteins in the ordinate. e. Purification from the nickel-limiting cells (50 nM Ni2+), from disruption of the cells through the three chromatographic steps, was completed within 1 day. f, Re-chromatography of the 1-MDa fraction from panel e after 40 h storage on ice. Although stability testing of the Elp-Hdr-Fmd complex of the purified 1-MDa complex was only conducted once, the elution profiles from the multiple purification processes, which differed in terms of the size of the 1-MDa peak, support that the complex is unstable.
