Extended Data Fig. 1: Variations of the sequence D-TLKIVWX (X = A, C, S, D, I, V, R, K, E, P or T) show varying efficacy in disassembling AD-tau.
From: How short peptides disassemble tau fibrils in Alzheimer’s disease
a, ThT assay of 20 μM tau K18+ fibrils added with H2O, 50 μM D-TLKIVWC, a mix of 50 μM D-TLKIVWC and 100 μM glutathione (GSH), or a mix of 50 μM D-TLKIVWC and 100 μM Glutathione disulfide (GSSG), respectively. b, Dot blot staining of D-TLKIVW, D-TLKIVWX (X = C, A, S, D, I, V, R, K, E, P or T) alone using the GT38 antibody. AD-tau was stained as a positive control. c, Representative TEM images of AD-tau after incubation with 500 μM D-TLKIVW or D-TLKIVWX (X = C, A, D, V, K, E, P or T) at 37 °C for 48 h. The AD-tau and newly formed fibrils are labelled with red and blue arrows, respectively. Scale bars, 500 nm. d, Quantification of AD-tau in panels c (n = 12 TEM images per group). Error bars represent mean + s.d.
