Extended Data Fig. 5: Related to Fig. 2: TUNEL staining in the brains of untreated and 4-HMA-treated Hpdl−/− mice.
From: Coenzyme Q headgroup intermediates can ameliorate a mitochondrial encephalopathy
a, Staining for Hoechst (blue) and TUNEL (yellow) showing the forebrain of Hpdl+/+, Hpdl−/− and Hpdl−/− + 4-HMA mice at P6 (panels A-C). The white dashed lines delineate the approximate areas of the cortex, striatum, thalamus and hippocampus that were quantified. Magnified view of the red-boxed areas are on the right (panels D-O). n = 3 slides per mouse and 3 mice/genotype. The graphs represent the density of TUNEL+ particles per mm squared in the cortex (panel P), striatum (panel Q), thalamus (panel R) and hippocampus (panel S) of the Hpdl+/+, Hpdl−/− and Hpdl−/− + 4-HMA mice at P6. For panels P-S, significance was tested by two-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test.
