Fig. 1: The transcriptional response of dFBNs to sleep deprivation.
a, Uniform manifold approximation and projection (UMAP) representation of glutamatergic neurons (grey) according to their gene expression profiles. dFBNs (purple) form a distinct cluster containing cells from rested (blue, n = 237 cells) and sleep-deprived brains (red, n = 86 cells). b, log-normalized expression levels of dFBN markers. c, Volcano plot of sleep history-dependent gene expression changes in dFBNs. Signals with Bonferroni-corrected P < 0.05 (two-sided Wilcoxon rank-sum test) are indicated in black; labels identify protein products localized to synapses or mitochondria; colours denote subunits of mitochondrial respiratory complexes. The P value of unc-13 exceeds the y-axis limit and is plotted at the top of the graph. d, Enrichment of the top ten downregulated (left) and upregulated (right) ‘biological process’ gene ontology terms in the set of differentially expressed dFBN genes.
