Extended Data Fig. 6: Sleep history does not alter the morphology of PN mitochondria.
a, b, Volumetric renderings (a) and morphometric parameters (b) of automatically detected mitochondria in OPRM image stacks of PN dendrites in rested and sleep-deprived flies. Mitochondrial number (P = 0.7077, two-sided t-test), volume (P = 0.8074, two-sided t-test), sphericity (P = 0.6500, two-sided t-test), and branch length (P = 0.5326, two-sided t-test) are unaffected by sleep deprivation. c, d, Maximum intensity projections (c) and morphometric parameters (d) of automatically detected mitochondria in CLSM image stacks of PN dendrites in rested and sleep-deprived flies. Mitochondrial number (P = 0.2534, two-sided Mann-Whitney test), volume (P = 0.7637, two-sided Mann-Whitney test), sphericity (P = 0.1953, two-sided Mann-Whitney test), and branch length (P = 0.6972, two-sided t-test) are unaffected by sleep deprivation. One data point exceeding the y-axis limits is plotted as a triangle at the top of the right-hand graph; mean and s.e.m. are based on the actual values. Scale bars, 10 µm (a), 20 µm (c). For statistical details see Supplementary Table 2.
