Extended Data Fig. 7: Morphological and metabolic consequences of inducing mitochondrial fission or fusion in dFBNs.
a, b, Volumetric renderings (a) and morphometric parameters (b) of automatically detected mitochondria in OPRM image stacks of dFBN dendrites. Flies carried R23E10-GAL4-driven overexpression constructs or RNAi transgenes targeting mitochondrial fission or fusion machinery. Manipulations that increase fission (green) or fusion (blue) have opposite effects on mitochondrial volume (P ≤ 0.0480, Holm-Šídák test after ANOVA), sphericity (P ≤ 0.0344, Holm-Šídák test after ANOVA), and branch length (P ≤ 0.0326, Holm-Šídák test after ANOVA). c, Morphometric parameters of automatically detected mitochondria in CLSM image stacks of dFBN dendrites. Flies carried R23E10-GAL4-driven overexpression constructs or RNAi transgenes targeting mitochondrial fission or fusion machinery. Manipulations that increase fission (green) or fusion (blue) have opposite effects on mitochondrial volume (P ≤ 0.0062, Dunn’s test after Kruskal-Wallis ANOVA), sphericity (P ≤ 0.0170, Holm-Šídák test after ANOVA), and branch length (P ≤ 0.0427, Dunn’s test after Kruskal-Wallis ANOVA). Five data points exceeding the y-axis limits are plotted as triangles at the top of the graphs; mean and s.e.m. are based on the actual values. d, Summed-intensity projections of dFBN dendrites expressing Drp1 and iATPSnFR plus RFP, in rested and sleep-deprived (SD) flies. Emission ratios are intensity-coded according to the key below and reduced in dFBNs expressing Drp1, irrespective of sleep history (Drp1 effect: P < 0.0001, sleep history effect: P < 0.0001, Drp1 × sleep history interaction: P = 0.1112; two-way ANOVA). Data are means ± s.e.m.; n, number of dendritic fields; asterisks, significant differences (P < 0.05) from both manipulations increasing fission. Scale bars, 10 µm (a), 5 µm (d). For statistical details see Supplementary Table 2.
