Extended Data Fig. 8: Inducing mitochondrial fission or fusion in dFBNs alters sleep.
a, b, Sleep in flies expressing R23E10-GAL4-driven fission or fusion proteins, or RNAi transgenes targeting transcripts encoding these proteins (a) or proteins regulating phosphatidic acid levels (b), and their parental controls. With the exception of the overexpression of Marf alone (P ≥ 0.1622, Holm-Šídák test after ANOVA), manipulations that increase fission (green) or fusion (blue) alter sleep in opposite directions (GTPases: P ≤ 0.0115, phosphatidic acid regulators: P ≤ 0.0381; Holm-Šídák test after ANOVA). c–e, Sleep in flies carrying R23E10-GAL4-driven Drp1 overexpression constructs or RNAi transgenes targeting mitochondrial fusion proteins not included in a, b and Fig. 4c: two independent constructs for Drp1 overexpression (c, P ≤ 0.0209, Dunn’s test after ANOVA); six independent RNAi transgenes directed against Opa1 (d, P ≤ 0.0199, Holm-Šídák test after ANOVA); and five independent RNAi transgenes directed against Marf (e, P ≥ 0.1017 relative to ≥1 parental control with the exception of R23E10 > Marf sm(II), Holm-Šídák test after ANOVA). f, Manipulations that increase fission (R23E10-GAL4 > Opa1RNAi, green) or fusion (R23E10 > Marf,Opa1, blue) alter the time courses (left panels, genotype effects: P ≤ 0.0213, time × genotype interactions: P < 0.0001, two-way repeated-measures ANOVA) and percentages of sleep rebound after deprivation (SD) in opposite directions (right panel, genotype effect: P ≤ 0.0186, Dunn’s test after ANOVA). One data point exceeding the y-axis limits is plotted as a triangle at the bottom of the right-hand graph; mean and s.e.m. are based on the actual values. g, Sleep in flies carrying an R23E10-GAL4-driven Marf overexpression construct not included in a and Fig. 4c (P ≥ 0.1252, Dunn’s test after Kruskal-Wallis ANOVA). Data are means ± s.e.m.; n, number of flies; asterisks, significant differences (P < 0.05) from both parental controls. For statistical details see Supplementary Table 2.
