Extended Data Fig. 2: Gene ontology analysis of sleep history-dependent gene expression in dFBNs.

a–d, Enrichment of gene ontology (GO) ‘biological process’ (a, c) and ‘cellular component’ (b, d) terms in the set of genes whose expression in dFBNs varies with sleep history. Panels a and b plot the top ten enriched terms by PANTHER Overrepresentation Test (fold enrichments >100 are truncated). Panels c and d show heatmaps (computed with ViSEAGO and topGO) of GO terms attached to downregulated (left) and upregulated (right) differentially expressed genes. Dendrograms represent semantic groupings among GO terms. P-values are colour-coded according to the keys on the right. e, Enrichment (top) and uncorrected P-values (bottom, dotted lines at P = 0.05) of the ‘cellular component’ GO terms ‘mitochondrion’ and ‘synapse’ in the sets of up- and downregulated genes by PANTHER Overrepresentation Test, in the full data set (dark grey) and after randomly downsampling (light grey) the number of rested dFBN transcriptomes to the number of sleep-deprived dFBN transcriptomes (n = 86 cells in either condition). The downsampling process was repeated ten times using the ‘max.cells.per.ident’ argument of the ‘FindMarkers’ function in Seurat, with reproducible seedings from 1 to 10.