Extended Data Fig. 3: Sleep history-dependent gene expression in Kenyon cells, projection neurons and non-dFBN cells.
a, UMAP representation of projection neurons (PNs, n = 317) from rested (blue) and sleep-deprived brains (red) according to their gene expression profiles. b, Volcano plot of sleep history-dependent gene expression changes in PNs. A single signal with Bonferroni-corrected P < 0.05 (two-sided Wilcoxon rank-sum test) is indicated in black. c, d, PANTHER Overrepresentation Test fails to detect enriched ‘biological process’ (g) and ‘cellular component’ (h) gene ontology (GO) terms in the set of differentially expressed PN genes. e, UMAP representation of Kenyon cells (KCs, n = 603) from rested (blue) and sleep-deprived brains (red) according to their gene expression profiles. f, Volcano plot of sleep history-dependent gene expression changes in PNs. Signals with Bonferroni-corrected P < 0.05 (two-sided Wilcoxon rank-sum test) are indicated in black. g, h, Enrichment of the top ten downregulated and upregulated ‘biological process’ (g) and ‘cellular component’ (h) GO terms in the set of differentially expressed KC genes by PANTHER Overrepresentation Test (fold enrichments >100 are truncated). i, j, Enrichment of the top ten downregulated and upregulated ‘biological process’ (i) and ‘cellular component’ (j) GO terms in the set of genes with differential expression in all non-dFBN cells by PANTHER Overrepresentation Test.
