Fig. 1: Haplotype-resolved assembly of diploid and tetraploid H. bulbosum genomes.
From: A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum
a, Diploid Hi-C contact matrix of the FB19-011-3 genome assembly. b, Graphical genotypes constructed from single-nucleus sequence data of FB19-011-3 pollen. Blue colour, haplotype 1; orange colour, haplotype 2. c, Haplotype-specific oligonucleotide-based FISH in mitotic chromosomes of FB19-011-3. The red and green probes target haplotypes 1 and 2, respectively, of chromosome 5H. At least two independent experiments were carried out to confirm the reproducibility of the labelling patterns. Scale bar, 10 μm. d, Hi-C contact matrix of the sequence assembly of the tetraploid clone FB19-028-3. e, Haplotype-specific oligonucleotide-based FISH in mitotic chromosomes of FB19-028-3. The red, green, blue and yellow probes target haplotypes 1, 2, 3 and 4, respectively, of chromosome 5H. At least two independent experiments were carried out to confirm the reproducibility of the labelling patterns. Scale bar, 10 μm.
