Extended Data Fig. 2: Extra-terminal crossovers occur in H. bulbosum more often than in H. vulgare.
From: A haplotype-resolved pangenome of the barley wild relative Hordeum bulbosum
(a) Representative examples of ring bivalents with interstitial (i), subterminal (s), terminal (t) and extra-terminal (et) chiasmata as calculated in (b). (b) Proportion of each type of chiasma based on the classification shown in (a). Number of cells scored from three independent plants: Barke: 110; Morex: 140; FB19_011_3: 120; FB20_005_1: 112; FB20_029_7: 104. (c) Representative examples of meiotic cells at metaphase I stage of H. vulgare cultivars “Barke” and “Morex” and H. bulbosum genotype FB19-011-3 showing different bivalent configurations and chiasma positions as calculated in b. (d) FISH with 5S and 45S rDNA (green) and HvT01 (subtelomeric; red) on metaphase I cells. 26 cells analyzed from two independent plants. One chromosome pair (arrowhead) showed extra-terminal chiasmata in 19 out of 26 cells analysed. Asterisks: extra-terminal chiasmata. Size bar = 10 μm.
