Extended Data Fig. 2: Anatomical considerations for mapping visual neurons throughout the medulla.
From: Eye structure shapes neuron function in Drosophila motion vision
Related to Fig. 2, a, Left: light microscopy of stochastically labelled Mi1 cells in the background of nc82 staining from a single substack showing the M10 layer of the medulla. Right: side view of stochastically labelled Mi1 cells (3 out of 4 columns have visible cells). b, A cross-section of the equatorial region in the lamina (from one eye of the FAFB EM volume), identified by cartridges (white contour) with 8 (orange) or fewer (yellow) outer photoreceptors. All equatorial columns corresponding to annotated lamina cartridges are indicated in Fig. 2c. c, A zoomed-in view of a single cartridge showing L1/2 cells and eight photoreceptor cells. L1 cells receive input from photoreceptor cells (R1-6) and output to Mi1 cells. d, 3D rendering of the same cartridge. e, Chiasm medulla columns (green) with corresponding Mi1 cells at three vertical locations identified by the twisting of R7 and R8 photoreceptor axons (not shown). For comparison, the central meridian is indicated in black. f, Extension of medulla column map to the lobula via interpolation of the positions of 63 reconstructed TmY5a neurons. g, The dendritic arbors of 114 reconstructed T4d cells in M10. The highlighted neurons are the examples in Fig. 2g.
